Agroforestry is a management program that intersperses trees within cropping systems, pastures, or grass buffers to provide economic benefits and ecosystem services. Previous studies have shown that agroforestry buffers can help reduce surface runoff, and sediment, nutrient and pesticide loss from row-crop agriculture. However, the potential of these buffers to mitigate antibiotic transport has yet to be investigated. Therefore, the goal of this research is to investigate the potential effects that agroforestry buffers may have for mitigating antibiotic transport from agroecosystems. The initial phase of this research involves investigating the sorption and desorption of oxytetracycline (tetracycline class), enrofloxacin (fluoroquinolone class), sulfadimethozine (sulfonamide class), and tylosin (macrolide class) to soils collected tree-grass buffers, grass buffers, and cropland. Results from adsorption/desorption isotherms, isotherm modeling, and correlation of isotherm model parameters with soil physical properties will be presented and discussed.